摘要
目的:建立RP-HPLC同时测定壮药清解雾化液中绿原酸、 芦丁、 金丝桃苷、 异槲皮苷4种指标成分的含量测定方法.方法:应用日本岛津LC-20A高效液相色谱仪,SPD-20A检测器,WondaCract ODS-2(4.6 mm×250 mm,5μm)色谱柱,以乙腈溶液(A)-0.2%磷酸水溶液(B)为流动相,梯度洗脱;流速1.0 mL/min,检测波长355 nm,柱温35℃.结果:绿原酸、 芦丁、 金丝桃苷、 异槲皮苷的进样量分别在2.22~5.55μg、0.330~0.825μg、0.208~0.52μg、0.3485~0.8713μg范围内线性关系良好(R2≥0.9997);加样回收率为97.5% ~102.35%,RSD值为1.15% ~2.14%.结论:该方法专属性、 稳定性、 重复性高,灵敏、 简便,可应用于壮药清解雾化液制备工艺质量控制.
Objective To develop an RP-HPLC method for simultaneous determination of chlorogenic acid,rutin,hyperoside and isoquercitrin in Qingjie Atomized Liquid.Methods Separation was carried out an WondaCract ODS-2(4.6 mm×250 mm,5μm),eluted with mobile phase of 0.2%phosphoric acid solution(B)-acetonitrile(A)in gradient mode.The flow rate was 1.0 mL/min.Column temperature was 35℃and detection wavelength was set at 355 nm.Result Linearity was good for chlorogenic acid,rutin,hyperoside and isoquercitrin in range of 2.22~5.55μg,0.330~0.825μg,0.208~0.52μg,0.3485~0.8713μg.respectively,Average recoveries of 4 compounds were 97.5%-102.35%,RSD(1.15%-2.14%).Conclusion The method is accurate,stable,repeatable,sensitive and simple,can be used for quality control of Qingjie Atomized Liquid.
作者
秦素红
莫小林
周雨晴
马家宝
杜沛霖
QIN Suhong;MO Xiaolin;ZHOU Yuqing;MA JiaBao;DU Peilin(The First Affiliated Hospital of Guangxi University of TCM,Nanning 530023,China;Guangxi University of Chinese Medicine,Nanning 530001,China)
出处
《中国民族民间医药》
2019年第13期61-63,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
广西壮族自治区卫生厅中医药科技专项制剂类课题资助项目和医院制剂遴选课题(GZYZ1104)
全国中药特色技术传承人才培训项目(J20164811007)
作者简介
秦素红(1988-),女,汉族,硕士,主管药师,研究方向中药新制剂、新剂型研发,E-mail:susu1437@163.com;通信作者:莫小林(1974-),女,汉族,本科,副主任中药师,研究方向为制剂开发和质量标准,E-mail:moxiaolin@163.com
