摘要
为了评估制样方法对高含糖量甜瓜样本轻稳定同位素分析的影响,本研究以甜瓜果皮、果肉匀浆样本为材料,分别采用热风喷粉干燥、真空冷冻干燥(-80℃,24 h)、真空微波干燥(2 min)、真空直接烘干(60℃,24 h)制备干燥样本,所有样本研磨制备精细粉末用于碳、氮、氢、氧稳定同位素比率(δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O)分析。对比4种方式制备样本在低温(-20℃)干燥箱存储60 d内重复测定结果的稳定性。结果表明,4种方式制备的高糖甜瓜果肉精细粉末极易吸湿潮解而干扰其δ^(2)H、δ^(18)O的测定,存储期长时样本值差异显著(P<0.05)。其中,真空冷冻干燥方式制备的瓜皮样本4种稳定同位素比率测定结果更稳定。真空冷冻干燥方式制样更易将甜瓜组织细胞壁破解后剩余的水分完全干燥,精细粉末样本在储藏过程中表现得更稳定,在不同储藏期的检测结果一致性高。通过对真空冷冻干燥方式制样的果皮样本稳定同位素比率进行偏最小二乘判别分析(PLS-DA)建模,区分新疆、广西、海南3个产地的58份甜瓜样本,判别准确度分别为100%、98.7%、89.1%。结果证明,真空冷冻干燥制备甜瓜果皮稳定同位素分析可以作为其产地确证的可靠方法,溯源模型可用于确证甜瓜原产地标签标识、保护地理标志产品。本研究为提升甜瓜产地溯源提供了新策略。
In order to evaluate the effects of different sample preparation methods on the analysis of light stable isotope ratios in sugar-rich sweet melon samples,melon peel and pulp samples were homogenized and pretreated by drying with hot air spray,vacuum freeze-drying(-80℃,24 h),vacuum microwave drying(for 2 min),vacuum direct drying(60℃,24 h).All dried samples were ground into fine powder for carbon,nitrogen,hydrogen,and oxygen stable isotope ratio analysis(δ^(13)C,δ^(15)N,δ^(2)H,δ^(18)O).Samples prepared by four pretreatment methods were stored in-20℃condition during 60 d to evaluate their effects on the origin traceability.It was found that the fine powder samples of sugar-rich melon pulp were easily hygroscopic and deliquescent,which interferes with the determination ofδ^(2)H andδ^(18)O.The values of samples during storage periods were significant different(P<0.05).Relatively,four stable isotope ratios in melon peel samples were more stable,and the vacuum freeze-drying method makes it easier to completely dry the remaining water after the cell wall of the melon tissue was cracked.Therefore,the fine powder sample was more stable during storage,and the test results at different storage periods were not significantly different(P>0.05).Based on the stable isotope ratios of the peel samples prepared by vacuum freeze-drying,the partial least squares discriminant analysis(PLS-DA)modeling was used to trace their origins for samples from Xinjiang,Guangxi and Hainan,and the discrimination accuracies were 100%,98.7%and 89.1%,respectively.The results prove that the stable isotope analysis of the peel prepared by vacuum freeze-drying can be used as a reliable method for the origin traceability of melon,and the traceability model can be used for the confirmation of its origin and to protect the geographical indication products.This study provides a new strategy for the traceability of melon.
作者
黎滢
刘志
范盈盈
何伟忠
赵多勇
张雁鸣
刘峰娟
王成
LI Ying;LIU Zhi;FAN Yingying;HE Weizhong;ZHAO Duoyong;ZHANG Yanming;LIU Fengjuan;WANG Cheng(College of Agriculture and Biotechnology,Hunan University of Humanities,Science and Technology,Loudi,Hunan 417000;Institute of Quality Standards&Testing Technology for Agro-Products/Laboratory of Quality and Safety Risk Assessment for Agro-Products,Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-products Quality and Safety of Xinjiang,Xinjiang Academy of Agricultural Sciences,Urumqi,Xinjiang 830091;Xinjiang Academy of Agricultural Sciences,Urumqi,Xinjiang 830091)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第S01期11-20,共10页
Journal of Nuclear Agricultural Sciences
基金
国家现代农业产业技术体系项目(CARS-25)
农产品质量安全与优质化业务技术委托任务(CSQA2020-05-13)
新疆维吾尔自治区天山英才计划(新人社函[2017]711号)
新疆维吾尔自治区天山雪松计划(2017XS07)
关键词
甜瓜
样本制备
PLS-DA建模
产地溯源
地理标志保护(PGI)
sweet melon
sample preparation
PLS-DA modeling
origin traceability
protection of geographical indications(PGI)
作者简介
黎滢,女,主要从事农产品质量安全与营养研究。E-mail:liying19990707@163.com;通讯作者:刘志,男,副教授,主要从事农产品质量安全与营养研究。E-mail:liuzhi19880122@126.com;通讯作者:王成,男,研究员,主要从事农产质量安全与控制研究。E-mail:wangcheng312@sina.com。
