摘要
目的探究艾滋筛查中HIV快速检测技术法(RT)与ELISA法的应用价值。方法开展回顾性分析法,遴选时段2019年6月—2020年6月内1500例HIV体抗筛查者,均接受快速检测技术法、ELISA法检测,以免疫印迹法(WB)结果为对照,分析检测结果,评估诊断价值。结果1500例筛查者,快速法阳性检出率1.47%(22/1500);ELISA法阳性检出率1.20%(18/1500);经免疫印迹法证实,阳性检出率为1.00%(15/1500);快速法敏感度为100.00%、特异度为99.53%、阳性预测值为68.18%、阴性预测值为100.00%;ELISA法敏感度为100.00%、特异度为99.80%、阳性预测值为83.33%、阴性预测值为100.00%。结论在HIV抗体检测中快速检测技术法、ELISA法均具有显著应用价值,两种检测方式敏感度、特异度无明显差异,故快速检测法均可用于艾滋筛查、诊断、检测咨询中。
Objective To explore the application of rapid HIV detection technology method(RT)versus ELISA method in HIV screening.Methods a retrospective analysis was conducted to select 1500 HIV somatic antibody screeners between June 2019 and June 2020 in the selection period,all of whom were tested by the rapid test technique,ELISA,and Western blot(WB)results were used as controls to analyze the test results and assess the diagnostic value.Results of 1500 screened individuals,the rapid method had a positive detection rate of 1.47%(22/1500);The positive detection rate of ELISA method was 1.20%(18/1500);The positive detection rate was1.00%(15/1500),as confirmed by immunoblotting;The rapid method had a sensitivity of 100.00%,specificity of99.53%,positive predictive value of 68.18%,and negative predictive value of 100.00%;The ECLIA assay had a sensitivity of 100.00%,specificity of 99.80%,positive predictive value of 83.33%and negative predictive value of100.00%.Conclusion the rapid test may be used in HIV screening,diagnosis,and testing counseling because it has significant application in HIV antibody testing,such that there is no significant difference in sensitivity or specificity between the two assays.
作者
蔡雅
Cai Ya(Department of Clinical Testing,Xuchang Xiangcheng County CDC,Xuchang,Henan,461700)
出处
《口岸卫生控制》
2022年第3期52-54,共3页
Port Health Control
作者简介
通信作者:蔡雅
