摘要
目的研究苦荞黄酮(TBF)对Ⅱ型糖尿病小鼠血糖水平的调控及其可能的机制。方法采用高糖高脂饮食联合链脲佐菌素(STZ)处理的方法复制Ⅱ型糖尿病小鼠模型,连续4周以苦荞黄酮灌胃,检测模型小鼠空腹血糖(FBG)和肝脏过氧化物酶体增殖剂激活受体γ(PPARγ)、磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶B(Akt)蛋白表达及Akt(Ser 473)磷酸化水平的变化。结果与对照组比较,TBF降低Ⅱ型糖尿病小鼠空腹血糖的效果差异有显著性(P<0.05),能显著提高PPARγ和PI3K的蛋白表达(P<0.01;P<0.05)以及Akt(Ser 473)的磷酸化水平(P<0.01)。结论TBF可能通过提高肝脏PPARγ,PI3K蛋白的表达及Akt磷酸化水平,改善Ⅱ型糖尿病。
Objective To study the effects of tatary buckwheat flavonoid(TBF)on expression and phosphorylation of PPARγ-PI3K/Akt pathway in T2DM mice.Methods The models of T2DM mice were produced by intraperitoneal injection of streptozotocin and feeding with high glucose and high fat diet.Mice were administered with TBF once a day for consecutive 4 weeks.The fasting blood-glucose was measured once a week.Western-blot was used to assess the expression of peroxisome proliferators-activated receptorγ(PPARγ),phosphatidylinositol 3-hydroxy kinase(PI3K),protein kinase B(Akt)and phosphorylated Akt in liver.Results Compared to model group,in TBF treated mice,the reduction of fasting blood-glucose was associated with remarkable increase of PPARγ,PI3K and phosphorylated Akt.Conclusion TBF alleviates the insulin resistance in T2DM mice by increasing the expression of PPARγ-PI3K/Akt related proteins.
作者
杨思
王菲
王晶
王子逸
陈丽梅
YANG Si;WANG Fei;WANG Jing;WANG Ziyi;CHEN Limei(School of Bioscience and Technology,Weifang Medical University,Weifang 261053,China)
出处
《潍坊医学院学报》
2020年第1期69-71,82,共4页
Acta Academiae Medicinae Weifang
基金
国家大学生创新创业训练计划项目(项目编号:201710438045)
作者简介
杨思(1997年~),女,学士学位。主要研究方向:生物制药研究;通讯作者:陈丽梅,E-mail:chenlim@wfmc.edu.cn
