摘要
目的评价多重PCR检测技术在诊断儿童急性呼吸道病毒感染中的应用价值,分析临床症状表现和检测到的相应病原体之间的相互联系。方法2018年8月至2019年8月采集成都市儿童专科医院275例有急性呼吸道感染症状的患儿咽拭子样本,使用RespiFinder~?SMART 22检测试剂盒进行检测。该试剂盒是基于多重PCR技术,可以一次性检测并区分22种有关呼吸道感染的病原体。结果275份样本中有245份(89.1%)检测出病毒感染为阳性,其中160份为单一病毒感染,59份为多重病毒感染,17份为肺炎支原体阳性,9份为肺炎衣原体阳性。最常见的是鼻病毒77份(28.0%)、腺病毒45份(16.4%)和呼吸道合胞病毒34份(12.4%)。在28份(10.2%)样本中检测出流感病毒。检出副流感25份(9.1%),冠状病毒15份(5.5%),博卡病毒12份(4.4%),人偏肺病毒9份(3.3%)。鼻病毒(28.0%)是上呼吸道感染中最常见的病原体,而呼吸道合胞病毒(12.4%)是下呼吸道感染中最常见的病原体。患儿的多重病毒感染与其临床表现的严重呼吸道症状有一定的关联性。结论运用多重PCR技术可以在实验检测中一次性检测多种病原体,较之普通单重PCR检测大大缩短了检测时间,对于快速诊断呼吸道多重病原体感染有着重要的临床意义。
Objective The aim of the study was to evaluate a multiplex molecular real-time assay for the diagnosis of respiratory viruses of childhood acute respiratory virus infection,and analyze the symptoms associated with the pathogens which were detected from those samples.Methods From August 2018 to August 2019,a sample of 275 swallow swabs from children with acute respiratory infections in Chengdu Children′s Specialist Hospital was collected and tested using the RespiFinder~?SMART 22 test kit.The kit is based on multiplex PCR technology and can detect and distinguish 22 pathogens related to respiratory infections at one time.Results Of the 275 samples,245(89.1%)detected positive viral infections,of which 160 were single virus infections,59 were multiple viral infections,17 were positive for Mycoplasma pneumoniae,and 9 were positive for Chlamydia pneumoniae.The most common pathogens were rhinovirus 77 cases(28.0%),adenovirus 45 cases(16.4%)and respiratory syncytial virus 34 cases(12.4%).Influenza virus was detected in 28(10.2%)samples,parainfluenza in 25 cases(9.1%),coronavirus in 15 cases(5.5%),Boca virus in 12 cases(4.4%),and human metapneumovirus in 9 cases(3.3%).Rhinovirus(28.0%)was the most common pathogen in upper respiratory tract infections,while respiratory syncytial virus(12.4%)was the most common pathogen in lower respiratory tract infections.It has been found that multiple viral infections in children were associated with severe respiratory symptoms in clinical manifestations.Conclusion Compared with ordinary PCR detection,the multiplex PCR could detect multiple pathogens at one time in experiment,which greatly shortens the detection time and had important clinical significance for rapid diagnosis of multiple respiratory pathogens.Thus,it could be useful for detecting several viruses causing respiratory tract disorders.
作者
徐佳楠
潘明
李天舒
童文彬
何树森
杨慧萍
XU Jia nan;PAN Ming;LI Tian shu;TONG Wen bin;HE Shu sen;YANG Hui ping(Institute of Microbiological Detection,Sichuan Province Center for Disease Control and Prevention,Chengdu,Sichuan 610041,China)
出处
《热带医学杂志》
CAS
2019年第12期1468-1471,1476,共5页
Journal of Tropical Medicine
基金
“十三五”国家科技重大专项(2017ZX10103010-006).
关键词
呼吸道感染
多重PCR
儿童
Respiratory tract infection
Multiplex PCR
Children
作者简介
徐佳楠(1981-),女,博士,主管技师,从事流感病毒等呼吸道病毒的实验室检测;通讯作者:杨慧萍,E mail:lydia_ping106@163.com
