摘要
目的建立蛤蚧定喘丸中龟鳖类成分的检测方法。方法借鉴动物类药材特征肽鉴别方法,采用胰蛋白酶对不同批次的蛤蚧定喘丸样品进行酶解,利用超高效液相色谱-质谱(UPLC-MS/MS)法,选择龟鳖类特征分子离子峰作为检测离子对,ESI+模式,进行多反应监测模式检测。结果8家生产企业共51批蛤蚧定喘丸样品中均检出鳖甲成分,未检出其他龟鳖源成分。结论该方法专属性较好,可用于蛤蚧定喘丸中龟鳖类成分掺伪检测。
Objective To develop an ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)to identification of the feeding status of turtle and turtle shell components in different batches of Gejie Dingchuan Pills.Methods This study used the identification method of characteristic peptides of Animal medicine for reference,Enzymatic hydrolysis of different batches of Ge jie Ding chuan Pill samples using trypsin,and Identification by using the ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The characteristic ion pairs of Testudinis Carapax et Plastrum and Trionycis Carapax were as detection ion pairs.The positive electrospray ionization and multiple reaction monitoring were adopted.Result:51 batches of Gejie Ding chuan Pills form 8 companies have all detected turtle shell components,but no other turtle source components have been detected.Conclusion This method is raliable for identification of Testudinis Carapax et Plastrum and Trionycis Carapax form Ge jie Ding chuan Pill.
作者
殷红军
吴越
华小懿
丁晴
张瑾
YIN Hong-jun;WU Yue;HUA Xiao-Yi;DING Qing;ZHANG Jin(Wuxi Instituteof Drug Safety Control,Wuxi 21400,China)
出处
《实验室检测》
2024年第2期10-13,共4页
Laboratory Testing
基金
江苏省市场监督管理局科技计划项目《动物药和矿物药质量控制的研究》项目编号:KJ2023044项目
中国药品监管科学行动计划第二批重点项目《中药有效性安全性评价及全过程质量控制研究》,课题名称:龟鳖类药材与饮片质量控制和评价研究,课题编号:NMPAJGKX-2023-045
关键词
蛤蚧定喘丸
鳖甲
龟甲
特征肽
超高效液相色谱-质谱
Gejie Ding chuan Pill
Testudinis Carapax et Plastrum
Trionycis Carapax
characteristic ion
UPLC-MS/MS
作者简介
通信作者:张瑾,硕士研究生,副主任中药师,研究方向为中药真伪鉴别与质量评价。E-mail:yinspire@126.com;殷红军,硕士,药物化学专业,主管药师,中药检验员,研究方向为中药检验。
