摘要
目的:比较外周血结核感染T细胞检测、抗酸染色和实时荧光聚合酶链反应法(qRT-PCR)检测结核分枝杆菌的灵敏度,探索临床检出结核分枝杆菌的更优方案。方法:选取临床确诊的80例肺结核患者,应用抗酸染色法及qRT-PCR回顾性检测其纤维支气管镜活检标本,并与其外周血结核感染T细胞检测结果相比较。结果:80例临床确诊肺结核病例中,传统的外周血结核感染T细胞检测阳性66例,qRT-PCR检测阳性76例,抗酸染色检测阳性75例。抗酸染色和qRT-PCR检测的灵敏度高于外周血结核感染T细胞检测,差异均有统计学意义(均P<0.05),联用抗酸染色和qRT-PCR检测结核分枝杆菌阳性78例,在其单独运用的基础上又进一步提高了灵敏度。结论:经纤维支气管镜活检取出的有限标本中,若标本充足应先行灵敏度较高qRT-PCR检测,联合抗酸染色可使灵敏度进一步提高。
Objective:To explore a better solution for clinical detection of mycobacterium tuberculosis by using the combination of T-SPOT.TB,Ziehl-Neelsen and fluorescent quantitative polymerase chain reaction(qRT-PCR).Methods:Ziehl-Neelsen and qRT-PCR were used to detect mycobacterium tuberculosis in 80 patients with clinical diagnosis of pulmonary tuberculosis,the results were compared with T-SPOT.TB.Results:Among the 80 cases of clinical confirmed tuberculosis,66 cases were positive by the traditional T-SPOT.TB,76 cases were positive by qRT-PCR,75 cases were positive by Ziehl-Neelsen.The sensitivity of Ziehl-Neelsen and qRT-PCR detection was higher than that T-spot.TB,and the difference was statistically significant(P<0.05),78 cases of mycobacterium tuberculosis were positive by Ziehl-Neelsen and qRT-PCR,the sensitivity was higher than that using them alone.Conclusion:Limited by bronchoscopic biopsy specimens of fibers withdrawn,qRT-PCR should be performed first,and the sensitivity can be further improved if combined with Ziehl-Neelsen.
作者
曹晓卉
沈丽华
杨志慧
梅佳
殷正进
孙桂凤
张缨
CAO Xiaohui;SHEN Lihua;YANG Zhihui;MEI Jia;YIN Zhengjin;SUN Guifeng;ZHANG Ying(Department of Pathology,General Hospital of Eastern Theater Command,Nanjing 210002,China;Department of Pathology,the Affiliated Brain Hospital of Nanjing Medical University,Nanjing 210029,China)
出处
《现代医学》
2020年第12期1564-1567,共4页
Modern Medical Journal
作者简介
曹晓卉(1987),女,江苏沛县人,技师。E-mail:15895849924@163.com;通信作者:张缨,E-mail:1738876035@qq.com
