摘要
目的:探讨生熟大黄药对对顺铂所致肾损伤小鼠的影响。方法:将40只小鼠随机分为生熟大黄高、中、低剂量组及模型对照组、空白对照组,每组8只,生熟大黄高、中、低剂量组及模型对照组以顺铂4mg/kg单次腹腔注射制作肾损伤模型,空白对照组不作特殊处理,生熟大黄高、中、低剂量组分别在给顺铂的同时予浓度分别为60mg/mL、120mg/mL及240mg/mL的生熟大黄水煎液灌胃,模型对照组给予同体积生理盐水灌胃,给药2周后取小鼠肾脏及血清。通过肾脏HE染色切片观察其肾小管及其上皮细胞形态改变。ELISA检测血清肌酐、尿素氮及血清胱抑素C的含量。结果:生熟大黄各治疗组与模型对照组比较血清肌酐、尿素氮及血清胱抑素C均下降,差异具有统计学意义(P<0.05)。肾脏的HE染色的病理学观察:生熟大黄水煎液能减轻顺铂所致肾小管上皮细胞的空泡化、基底膜增厚及皱缩,同时改善肾间质的炎性浸润。结论:生熟大黄药对对小鼠顺铂所致肾损伤具有改善作用。
Objective:To investigate the effect of raw and cooked rhubarb on cisplatin-induced kidney injury in mice.Methods:Forty mice were randomLy divided into high,middle and low dose groups of raw and cooked rhubarb,model control group and blank control group,with 8 mice in each group.The renal injury model was made by single intraperitoneal injection of cisplatin 4 mg/kg in high,middle and low dose groups of raw and cooked rhubarb and model control group,while no special treatment was given in blank control group.The high,middle and low dose groups of raw and cooked rhubarb were given cisplatin while the decoction of raw and cooked rhubarb with the concentration of 60 mg/mL,120 mg/mL and 240 mg/mL was given to the stomach,while the model control group was given the same volume of normal saline.Two weeks later,the kidneys and serum of mice were taken.The morphological changes of renal tubules and epithelial cells were observed by HE staining sections of kidney.ELISA was used to detect serum creatinine,urea nitrogen and serum cystatin C content.Results:Compared with the model control group,the serum creatinine,urea nitrogen and serum cystatin C in each treatment group of raw and cooked rhubarb decreased,with statistically significant difference(P<0.05).Pathological observation of HE staining of kidney:raw and cooked rhubarb decoction can reduce vacuolation,basement membrane thickening and shrinkage of renal tubular epithelial cells caused by cisplatin,and improve inflammatory infiltration of renal interstitium at the same time.Conclusion:Raw and cooked rhubarb drugs can improve kidney injury induced by cisplatin in mice.
作者
琚皇进
杨柱
唐东昕
罗莉
龙奉玺
王镜辉
郭斌
Ju Huangjin;Yang Zhu;Tang Dongxin;Luo Li;Long Fengxi;Wang Jinghui;Guo Bin(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China;National Traditional Chinese Medicine Hospital of Dejiang County,Tongren565200,China)
出处
《亚太传统医药》
2020年第11期24-26,共3页
Asia-Pacific Traditional Medicine
基金
国家自然科学基金(81673863、81660833、81760814)
贵州省科技厅项目(黔科合人才(2016)4032号)
贵州省教育厅项目(黔教研合GZS字[2016]08)
贵州省委组织部项目(黔人领发[2018]3号)
贵州省中医药管理局项目(QZYY-2018-014)
关键词
肾损伤
生熟大黄药对
顺铂
小鼠实验
Kidney Injury
Raw and Cooked Rhubarb Drug Pairs
Cisplatin
Mouse Experiment
作者简介
琚皇进(1991-),男,硕士,贵州省德江县民族中医院主治医师,研究方向为中医基础理论在肿瘤防治中的运用;通讯作者:唐东昕(1977-),男,贵州中医药大学第一附属医院教授,博士生导师,研究方向为中医药防治肿瘤的基础及临床。E-mail:tangdongxin@sina.com
