摘要
SARS-CoV-2 has caused tens of thousands of infections and more than one thousand deaths.There are currently no registered therapies for treating coronavirus infections.Because of time consuming process of new drug development,drug repositioning may be the only solution to the epidemic of sudden infectious diseases.We systematically analyzed all the proteins encoded by SARS-CoV-2 genes,compared them with proteins from other coronavirnses,predicted their structures,and built 19 structures that could be done by homology modeling.By performing target-based virtual ligand screening,a total of21 targets(including two human targets)were screened against compound libraries including ZINC drug database and our own database of natural products.Structure and screening results of important targets such as 3-chymotrypsin-like protease(3 CLpro),Spike,RNA-dependent RNA polymerase(RdRp),and papain like protease(PLpro)were discussed in detail.In addition,a database of 78 commonly used antiviral drugs including those currently on the market and undergoing clinical trials for SARS-CoV-2 was constructed.Possible targets of these compounds and potential drugs acting on a certain target were predicted.This study will provide new lead compounds and targets for further in vitro and in vivo studies of SARS-CoV-2,new insights for those drugs currently ongoing clinical studies,and also possible new strategies for drug repositioning to treat SARS-CoV-2 infections.
基金
support from National Mega-project for Innovative Drugs(grant number 2019ZX09721001-004-007,China)
National Natural Science Foundation of China(NSFC,grant numbers U1803122,81773637,81773594,and U1703111)
the Fundamental Research Fund for the Central Universities(HUST COVID-19 Rapid Response Call,No.2020kfyXGYJ037,China)
作者简介
Corresponding author:Xingzhou Li,E-mail addresses:xingzhouli@aliyun.com;Corresponding author:Mengzhu Zheng,mengzhu_zheng@hust.edu.cn;Corresponding author:Lixia Chen,syzyclx@163.com,Corresponding author:Hua Li,li_hua@hust.edu.cn;-Canrong Wu,Yang Liu,Yueying Yang,These authors made equal contributions to this work.
