摘要
目的探讨S-腺苷甲硫氨酸(S-adenosyl-l-methionine,SAM)对戒烟肺气肿小鼠氧化应激、肺泡隔细胞凋亡的影响。方法将32只6~8周龄雄性SPF级C57BL/6小鼠随机分为4组:健康对照组、肺气肿组、戒烟组、SAM干预组,每组8只。使用烟草烟雾提取物(cigarette smoke extract,CSE)腹腔注射联合香烟烟雾暴露构建小鼠肺气肿模型,肺气肿模型构建成功后戒烟组和SAM干预组开始戒烟8周。SAM干预组小鼠在戒烟8周期间腹腔注射SAM 100 mg·kg^(-1)·d^(-1)。各组小鼠取右肺组织切片行苏木精-伊红染色观察病理形态学改变,并测定肺平均内衬间隔(mean linear intercept,MLI)、平均肺泡数(mean alveolar number,MAN)。左肺行肺泡灌洗,以分光光度剂检测肺泡灌洗液中的丙二醛(malondialde-hydecontent,MDA)、超氧化物歧化酶(superoxidedismutase,SOD)及谷胱甘肽(glutathione,GSH)浓度。采用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记技术检测肺泡隔细胞调亡。结果MLI、肺泡隔细胞凋亡指数及支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中MDA浓度比较:肺气肿组较健康对照组增加,戒烟组较肺气肿组增加,SAM干预组较戒烟组减少,差异均有统计学意义(P<0.05)。MAN及BALF中GSH浓度、SOD活力比较:肺气肿组较健康对照组减少,戒烟组较肺气肿组减少,SAM干预组较戒烟组增加,差异均有统计学意义(P<0.05)。结论戒烟肺气肿小鼠肺组织病理、肺泡隔细胞凋亡呈持续进展状态,SAM可能通过减轻氧化应激反应、改善肺泡隔细胞凋亡,从而达到保护戒烟小鼠肺气肿的目的。
Objective To investigate the effect of S-adenosyl-l-methionine(SAM)on oxidative stress and alveolar septal cell apoptosis in mice with emphysema after smoking cessation.Methods Twenty-two male SPF C57BL/6J mice aged 6-8 weeks were randomly divided into 4 groups,ie.a healthy control group,an emphysema group,a smoking cessation group,and a SAM intervention for 8 weeks after smoking cessation group,with 8 mice in each group.The mice model of emphysema was established by intraperitoneal injection of cigarette smoke extract(CSE)combined with cigarette smoke exposure.Smoking cessation started after the emphysema model was successfully constructed and lasted for 8 weeks.After smoking cessation,the mice in SAM intervention groups were intraperitoneally injected with SAM mg·kg^(-1)·d^(-1)for 8 weeks.The right lung sections of the mice were taken for hematoxylin-eosin staining to observe pathological changes,and the mean linea rintercept(MLI)and mean alveola rnumber(MAN)of lungs were measured.The concentrations of malondialdehyde(MDA),superoxide-dismutase(SOD)and glutathione(GSH)in alveolar lavage fluid of left lung were detected by spectrophotometry.Terminal deoxynucleotidyl transferasemediated dUTP nick end labeling(TUNEL)technique was carried out to detect the alveolar septal cells apoptosis.Results MLI,apoptosis index of alveolar septal cell and MDA concentration in bronchoalveolar lavage fluid(BALF)increased significantly in the emphysema group compared with healthy controls,increased significantly in the smoking cessation group compared with the emphysema group,and decreased in the SAM intervention group compared with the smoking cessation group(all P<0.05).GSH concentration and SOD activity in BALF and MAN was significantly lower in the emphysema group compared with the healthy control group,significantly lower in the smoking cessation group compared with the emphysema group,and significantly higher in the SAM intervention group compared with the smoking cessation group(all P<0.05).Conclusions Lung histopathology and apoptosis of alveolar septal cells in emphysema mice progress continuously after smoking cessation.SAM may reduce oxidative stress and improve apoptosis of alveolar septal cells,so as to protect emphysema mice after smoking cessation.
作者
张艳梅
张程
ZHANG Yanmei;ZHANG Cheng(School of Clinical Medicine,Zunyi Medical University,Zunyi,Guizhou 563000,P.R.China;Department of Respiratory and Critical Care Medicine,Guizhou Provincial People's Hospital,Guiyang,Guizhou 550002,P.R.China;NHC Key Laboratory of Pulmonary Immunological Diseases,Guizhou Provincial People's Hospital,Guiyang,Guizhou,China)
出处
《中国呼吸与危重监护杂志》
CAS
CSCD
2024年第11期797-801,共5页
Chinese Journal of Respiratory and Critical Care Medicine
基金
贵州省科技计划项目(黔科合基础-ZK[2021]一般350)
贵州省卫健委2020(No.gzwjkj2020--049)
关键词
肺气肿
氧化应激
S-腺苷甲硫氨酸
香烟烟雾
肺泡隔细胞
凋亡
Emphysema
oxidative stress
S-adenosine methionine
cigarette smoke
alveolar septal cells
apoptosis
作者简介
通信作者:张程,Email:zhangcheng16@sina.com
