摘要
虾虎鱼(Gobiidae)是海洋底层食物链中的关键环节,在生态系统的物质循环和能量流动中发挥重要作用。因虾虎鱼种类繁多、分布广泛、体型较小,加之形态上存在不同程度的特化,对形态学分类鉴定带来极大挑战。本研究对采自黄渤海的73尾虾虎鱼成鱼和幼鱼样本分别进行了形态学鉴定和DNA条形码分析,依据形态学分类特征,鉴定出9属12种;而运用DNA条形码技术,共鉴定出13种,隶属于虾虎鱼科10属。可见,在形态学鉴定经验不足或幼鱼形态学特征不明显的情况下,DNA条形码可以有效地实现物种鉴定。同时,系统整理了黄渤海虾虎鱼科记录种名录,共计29属47种。依据物种名录,从BOLD及NCBI数据库中筛选并下载了18属29种虾虎鱼科鱼类的DNA条形码,与本研究鉴定出的10属13种虾虎鱼类合并分析,构建了虾虎鱼科26属42种鱼类的系统关系树,覆盖了黄渤海虾虎鱼科89.36%的记录种,通过对虾虎鱼科鱼类的分子系统发育关系与形态学分类地位进行确认和修订,初步建立了黄渤海虾虎鱼科DNA条形码分类体系。本研究结果证明了DNA条形码技术对虾虎鱼物种具有较高识别效率,有效弥补了传统形态学鉴定方法的缺点和局限,丰富了虾虎鱼DNA条形码数据库,完善了虾虎鱼DNA条形码分类体系,为虾虎鱼的保护生物学、进化生物学及生物地理学等研究提供了科学依据。
The goby is a key link in the food chain at the bottom of the ocean and plays an important role in the material circulation and energy flow of the ecosystem.Due to the large variety,extensive distribution,and small size of the goby,as well as different degrees of degradation and specialization in morphology,it presents great challenges to morphological classification and identification.In this study,73 adult and juvenile goby samples from the Yellow Sea and Bohai Sea were identified by morphology and DNA barcoding.According to the morphological classification,12 species of 9 genera were identified,while 13 species were identified belonging to 10 genera of family Gobiidae.Thus,in the case of insufficient experience in morphological identification or unclear morphological characteristics of juvenile fish,DNA barcoding can effectively identify species.At the same time,the list of recorded species of family Gobiidae in the Bohai Sea and Yellow Sea of China was systematically sorted out,with a total of 47 species of 29 genera.According to the recorded species list,the DNA barcodes of 29species of 18 genera belonging to the family Gobiidae were screened and downloaded from the BOLD and NCBI databases.Combined with the 13 species of 10 genera of gobies identified in this study,a phylogenetic tree of 42species of 26 genera was constructed,covering 89.36%of the recorded species of gobies in the Bohai Sea and Yellow Sea of China.The DNA barcode classification system of gobies was established by confirming and revising the molecular phylogenetic relationship and morphological classification status of gobies.The results of this study demonstrate the recognition efficiency of DNA barcoding in the classification of the gobies,make up for the shortcomings and limitations of traditional morphological identification methods,enrich the goby DNA barcode database,improve the goby DNA barcoding classification system,and provide a scientific basis for research on the conservation biology,evolutionary biology,and biogeography of goby fish.
作者
李明晖
安长廷
李昂
刘凯莹
柳淑芳
庄志猛
LI Minghui;AN Changting;LI Ang;LIU Kaiying;LIU Shufang;ZHUANG Zhimeng(Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;Function Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2022年第8期1179-1188,共10页
Journal of Fishery Sciences of China
基金
国家重点研发计划蓝色粮仓项目(2018YFD0900803)
中国水产科学研究院基本科研业务费资助项目(2021JC01)
国家海洋水产种质资源库项目
关键词
虾虎鱼
DNA条形码
物种鉴定
系统发育关系
DNA条形码分类体系
gobies
DNA barcoding
species identification
molecular phylogenetic relationships
DNA barcode classification system
作者简介
李明晖(1996-),男,硕士研究生,研究方向为渔业资源分子生态学.E-mail:598545891@qq.com;通信作者:柳淑芳,研究员,研究方向为渔业资源分子生态学.E-mail:liusf@ysfri.ac.cn
