摘要
目的:测定怀牛膝及其芦头中25R-牛膝甾酮、25S-牛膝甾酮、蜕皮甾酮、人参皂苷R0、竹节参皂苷Ⅳa 5种成分的含量。方法:采用HPLC法测定怀牛膝中5种成分含量,并采用聚类分析及主成分分析法对其质量进行综合评价。结果:不同采收地怀牛膝、不同规格怀牛膝中人参皂苷R0、竹节参皂苷Ⅳa的含量差异较大,蜕皮甾酮、25R-牛膝甾酮、25S-牛膝甾酮含量差异较小;聚类分析和主成分分析均将不同采收地怀牛膝分为4类;芦头中蜕皮甾酮、25R-牛膝甾酮、人参皂苷R0、竹节参皂苷Ⅳa含量均高于药材,而25S-牛膝甾酮含量无明显差异;聚类分析能将药材及芦头区分开。结论:采收地、规格、芦头均对怀牛膝药材质量有一定影响。
Objective:To determine the contents of five components(25R-inokosterone,25S-inokosterone,ecdysterone,ginsenoside R0,chikusetsu saponin Ⅳa)in Achyranthis Bidentatae Radix and rhizome.Methods:HPLC method was used to determine the contents of five components in Achyranthis Bidentatae Radix,and the quality was comprehensively evaluated by cluster analysis and principal component analysis.Results:There was little difference in the contents of ecdysterone,25S-inokosterone and 25R-inokosterone in Achyranthis Bidentatae Radix from different areas and different specifications,while there was great difference in the contents of ginsenoside R0 and chikusetsu saponin Ⅳa;Cluster analysis and principal component analysis all divided Achyranthis Bidentatae Radix into four categories.The contents of ecdysterone,25R-inokosterone,ginsenoside R0,chikusetsu saponin Ⅳa in rhizome were higher than that in Achyranthis Bidentatae Radix,there was no significant difference in the content of 25S-inokosterone;Cluster analysis could distinguish the Achyranthis Bidentatae Radix from rhizome.Conclusion:The quality of Achyranthis Bidentatae Radix is affected by the field,specification and rhizome.
作者
梁献葵
雷敬卫
龚海燕
唐维维
纪亮
谢彩侠
刘东方
LIANG Xian-kui;LEI Jing-wei;GONG Hai-yan;TANG Wei-wei;JI Liang;XIE Cai-xia;LIU Dong-fang(Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中药材》
CAS
北大核心
2019年第7期1578-1583,共6页
Journal of Chinese Medicinal Materials
基金
国家重点研发计划“中医药现代化研究”重点专项项目(2018YFC1707000)
河南省科技攻关项目(162102310448).
关键词
怀牛膝
规格
芦头
含量测定
Achyranthis Bidentatae Radix Bl.
Specification
Rhizome
Content determination
作者简介
梁献葵(1991-),女,在读硕士研究生,专业方向:中药质量分析研究,E-mail:646909449@qq.com;通讯作者:雷敬卫,Tel:0371-656955281,E-mail:yjs2017tg@126.com。
