摘要
目的:通过网络药理学方法探究柴胡-葛根药对治疗由新型冠状病毒(2019-nCoV)引起的新型冠状病毒肺炎(COVID-19)的潜在作用机制,为后续机制及临床转化研究提供理论指导。方法:通过TCMSP在线平台获取柴胡与葛根的潜在活性成分及各自相关作用靶点,并利用GeneCards数据库检索COVID-19相关疾病靶点,将上述二者共有的靶点输入STRING蛋白互作在线数据库,从而获取潜在作用靶点的互作关系网络,并进一步通过MCODE插件筛选潜在核心靶点。利用Cytoscape 3.2.1软件构建“成分-靶标-疾病”网络与PPI网络,并通过DAVID v6.8在线软件进行GO功能富集分析与KEGG信号通路富集分析。结果:在柴胡-葛根药对中筛选得到了22个活性成分和226个药物作用靶点,其中与COVID-19共同作用的靶标有47个,潜在核心靶点有21个。GO功能富集分析结果表明主要与基因转录表达、炎性反应及免疫系统反应等相关,KEGG信号通路富集分析结果表明主要与甲型流感病毒、TNF通路及Toll样受体信号通路等相关。结论:柴胡-葛根药对能通过多成分、多靶点、多途径的作用方式,从炎症反应、免疫调节等方面发挥其抗2019-nCoV的作用。
Objective:To explore the potential action mechanism of Bupleuri Radix and Puerariae Lobatae Radix herb-pair in the treatment of Corona Virus Disease 2019(COVID-19)caused by 2019-nCoV virus through network pharmacology method,so as to provide theoretical guidance for further mechanism and clinical translational research.Methods:The potential active ingredients and their respective related action targets of Bupleuri Radix and Puerariae Lobatae Radix were obtained through the TCMSP,and the COVID-19-related disease targets were searched by GeneCards database.The common targets from the both screenings were input into the STRING protein interaction online database to construct the interaction network of potential targets.The potential core targets were further screened by MCODE plug-ins.The“ingredients-targets-diseases”network and PPI network were constructed by Cytoscape 3.2.1 software,and GO function enrichment analysis and KEGG signal pathway enrichment analysis were carried out by DAVID v6.8 online software.Results:A total of 22 active ingredients and 226 drug targets were screened from Bupleuri Radix and Puerariae Lobatae Radix herb-pair,among which 47 were co-acting targets with COVID-19 and 21 were potential core targets.The results of GO functional enrichment analysis showed that it was mainly related to gene transcriptional expression,inflammatory response and immune system response,while KEGG signal pathway enrichment analysis showed that it was mainly associated with influenza A virus,TNF pathway,Toll-like receptor signal pathway and others.Conclusion:Bupleuri Radix and Puerariae Lobatae Radix herb-pair can exert an anti-2019-nCoV effect through its regulatory role in inflammatory reaction and immune system with a multi-ingredients,multi-targets and multi-pathways pharmacological characteristics.
作者
潘博宇
曹鸿静
王耘
刘立仁
PAN Bo-yu;CAO Hong-jing;WANG Yun;LIU Li-ren(Department of Gastrointestinal Cancer Biology,Tianjin Medical University Cancer Institute&Hospital/Tianjin Medical University Cancer Institute&Hospital/National Clinical Research Center for Cancer/Key Laboratory of Cancer Prevention and Therapy,Tianjin/Tianjin′s Clinical Research Center for Cancer,Tianjin 300060,China;Tianjin Teda Hospital,Tianjin 300457,China;Department of Integrated Traditional&Western Medicine/Tianjin Medical University Cancer Institute&Hospital/National Clinical Research Center for Cancer/Key Laboratory of Cancer Prevention and Therapy,Tianjin/Tianjin′s Clinical Research Center for Cancer,Tianjin 300060,China)
出处
《中药材》
CAS
北大核心
2021年第2期495-503,共9页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金面上项目(81572416)
作者简介
潘博宇(1990-),男,硕士,研究实习员,研究方向:中药抗肿瘤药理机制,Tel:panboyu@tmu.edu.cn;通讯作者:刘立仁,liuliren@tmu.edu.cn。
