摘要
为了探讨高含固污泥热水解-中温厌氧消化切换至高温厌氧消化的产甲烷特性,研究分析高含固中温厌氧污泥在不同氨质量浓度和温度下乙酸、丙酸、丁酸及热水解污泥的产甲烷活性,并开展高温连续运行试验。结果显示,高含固热水解中温厌氧污泥对乙酸、丙酸、丁酸及热水解污泥的产甲烷活性均随温度升高和氨质量浓度增加而下降,高温环境下的氢利用速率和互营乙酸氧化速率受氨质量浓度影响较小。连续高温运行试验表明,水力停留时间(Hydraulic Retention Times,HRT)在20 d、15 d和10 d下连续运行反应器容积产气率稳定在(0.47±0.12)~(1.01±0.23)L/(L·d),挥发性脂肪酸质量浓度稳定在(85.90±9.40)~(100.42±7.85)g/L(以COD计)。在高温运行稳定阶段,氨氮质量浓度为3 g/L的条件下,HRT分别为10 d、15 d和20 d的反应器中,产甲烷活性均有不同程度的提升,氢利用速率分别提升44.62%、48.21%和45.74%。微生物种群结构分析表明,高含固污泥在高温厌氧消化系统中的优势产甲烷菌Methanothermobacter相对丰度最高可达85.10%,Methanosarcina为10.41%。虽然,在高温高氨环境下的乙酸产甲烷速率仅维持在切换前的23%~63%,但氢利用速率的强化保证了系统良好的产甲烷性能。综上,构建互营乙酸氧化-氢营养型产甲烷(Syntrophic Acetate Oxidation-Hydrogenotrophic Methanogenesis,SAO-HM)途径,对实现高含固热水解-高温厌氧消化稳定运行具有促进作用。
To investigate the methane production characteristics of high-solid sludge thermal hydrolysis followed by thermophilic anaerobic digestion,this study analyzed the methanogenic activity of inoculated sludge from high-solid sludge thermal hydrolysis and mesophilic anaerobic digestion under varying ammonia concentrations and temperatures.The analysis focused on acetic acid,propionic acid,butyric acid,and thermally hydrolyzed sludge,and included continuous thermophilic anaerobic digestion experiments.The results indicated that the methanogenic activity of the inoculated sludge decreased with increasing temperature and ammonia concentration for acetic acid,propionic acid,butyric acid,and thermally hydrolyzed sludge.However,under thermophilic conditions,the Hydrogen Utilization Rate(HUR)and the Syntrophic Acetate Oxidation(SAO)rate were less influenced by ammonia concentration.Continuous operation tests demonstrated that the volumetric biogas production rate of the reactor remained stable,ranging from(0.47±0.12)L/(L·d)to(1.01±0.23)L/(L·d).Additionally,the concentration of volatile fatty acids,expressed as COD,was consistently stable at(85.90±9.40)g/L to(100.42±7.85)g/L across Hydraulic Retention Times(HRT)of 20 d,15 d,and 10 d.During the stable operation phase of thermophilic anaerobic digestion,the methanogenic activity of digested sludge from reactors with HRT of 10 d,15 d,and 20 d at an ammonia concentration of 3 g/L NH+4 N increased to varying degrees for each substrate,resulting in increases in HUR of 44.62%,48.21%,and 45.74%,respectively.Analysis of the microbial community structure revealed that the dominant archaeal genera in the high-solid sludge thermal hydrolysis and thermophilic anaerobic digestion system were Methanothermobacter,which comprised up to 85.10%of the community,and Methanosarcina,which accounted for 10.41%.Although the methanogenic activity for acetic acid was only maintained at 23%to 63%of the pre-switching rate under high temperature and elevated ammonia levels,the increased HUR ensured the system's overall excellent methane production performance.In summary,the establishment of the Syntrophic Acetate Oxidation-Hydrotrophic Methanogenesis(SAO HM)pathway enhances the stable operation of high-solid sludge thermal hydrolysis followed by thermophilic anaerobic digestion.
作者
韩芸
王雪娜
朱俊兆
周梦雨
刘奕
王皓
王松
卓杨
HAN Yun;WANG Xuena;ZHU Junzhao;ZHOU Mengyu;LIU Yi;WANG Hao;WANG Song;ZHUO Yang(School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China;Key Laboratory of Northwest Water Resource,Environment and Ecology,MOE,Xi’an University of Architecture and Technology,Xi’an 710055,China)
出处
《安全与环境学报》
北大核心
2025年第2期694-705,共12页
Journal of Safety and Environment
基金
国家自然科学基金项目(52200175,52070153)
陕西省重点研发计划项目(2023-YBSF-283)。
关键词
环境工程学
高含固污泥
热水解
高温厌氧消化
氨抑制
environmental engineering
high solid sludge
thermal hydrolysis
thermophilic anaerobic digestion
ammonia inhibition
作者简介
韩芸,教授,博士,从事污泥处理与处置和污水再生利用研究,hanyun@xauat.edu.cn。
