摘要
为了筛选出对核桃腐烂病具有高效防治效果、同时对核桃种子发芽具有促生作用的生防菌株,从新疆温宿县核桃林场核桃园采集土壤,采用稀释涂布法进行生防菌的分离与筛选。通过形态学特征、生理生化特征以及分子生物学方法对所分离的菌株进行物种鉴定,并研究其抗菌能力、防病效果以及对核桃种子发芽的促进作用。从土壤中分离到一株具有广谱抑菌能力的链霉菌F-04(CGMCC No.29519),经过分类鉴定,确定为德干链霉菌(Streptomyes deccanensis)。该菌对核桃腐烂病菌Cytospora chrysosperma的抑菌率为81.75%;其发酵滤液对核桃枝条腐烂病的防治效果可达87.24%;此外,该菌株的发酵滤液对核桃种子发芽有显著促进作用,在150 mL·L^(-1)的浓度下,发芽率最高(85.32%),同时在该浓度处理下,坏种率最低(9.32%)。
To identify the biocontrol strains with high control effect on walnut rot and promote the germination of walnut seeds,soil was collected from the walnut orchard of a walnut forest farm in Wensu County,Xinjiang.Biocontrol bacteria were isolated and screened by a dilution coating method.The biocontrol bacteria were identified by morphological characteristics,physiological and biochemical characteristics combined with molecular biology,and their antibacterial ability,disease prevention effect and promoting effect on walnut seed germination were studied.A strain of Streptomyces F-04(CGMCC No.29519)with broad-spectrum antibacterial activity was isolated,and it was identified as Streptomyes deccanensis.The inhibitory rate of this strain against Cytospora chrysosperma was 81.75%,and its fermentation filtrate achieved an 87.24% control effect on walnut branch rot.At a concentration of 150 mg·mL^(-1),the fermentation filtrate resulted in the highest germination rate of 85.32%,while the seed failure rate was the lowest at 9.32%.This demonstrates a significant promoting effect on walnut seed germination.
作者
刘志金
石凌旭
张佳
乔杰栋
于雅雯
袁昊
张锐
张建良
陈小飞
LIU Zhijin;SHI Lingxu;ZHANG Jia;QIAO Jiedong;YU Yawen;YUAN Hao;ZHANG Rui;ZHANG Jianliang;CHEN Xiaofei(College of Agriculture,Tarim University/Key Laboratory of Integrated Pest Management(IPM)of Xinjiang Production and Construction Corps in Southern Xinjiang,Alar,Xinjiang 843300,China;College of Horticulture and Forestry,Tarim University,Alar,Xinjiang 843300,China;Agricultural Development Service Center of the Third Regiment of Alar City,the Division of Xinjiang Production and Construction Corps,Alar,Xinjiang 843011,China)
出处
《干旱地区农业研究》
CSCD
北大核心
2024年第6期216-224,共9页
Agricultural Research in the Arid Areas
基金
国家级林业科技成果推广项目[2019]55
国家自然科学基金项目(31560007)
新疆生产建设兵团“三区”人才支持计划科技人员专项计划项目(BT1720230048G)。
关键词
核桃腐烂病
生防放线菌
促生作用
walnut rot
biocontrol bacterium
growth promoting effect
作者简介
刘志金(1999-),男,青海互助人,硕士研究生,研究方向为植物病理学。E-mail:3115273782@qq.com;通信作者:陈小飞(1978-),男,湖南桃源人,教授,主要从事农作物病虫害绿色防控研究。E-mail:cxfzky@126.com。
