摘要
目的:探讨丙酮沉淀法提取杠板归多糖的最佳工艺条件。方法:以杠板归为研究对象,以水为溶剂提取杠板归多糖,提取液用丙酮进行沉淀,选取离心时间、沉淀时间和沉淀剂用量3个因素做单因素进行正交实验,探索最佳提取条件。结果:丙酮加入量75ml、沉淀时间18h和离心时间18min是丙酮沉淀法提取杠板归多糖的最佳工艺条件。结论:确定了丙酮沉淀法提取杠板归多糖的最佳工艺条件。
Objective： To study the best extraction conditions of polysaccharide from Polygonum perfoliatum by acetone deposition method.Methods： With Polygonum perfoliatum as research object and water as solvent,extraction of tea polysaccharide under certain conditions were precipitated with acetone.The choices level such as centrifugal time,the amount of acetone and precipitation time were made by single factor tests and orthogonal tests.The best extraction conditions were obtained by the experiments.Results： The best extraction conditions of acetone deposition method were： the amount of acetone being 75 ml,centrifugal time being 18 min,precipitation time being 18 h.Conclusion： The best extraction conditions of polysaccharide from Polygonum perfoliatum is determined by acetone deposition method.
出处
《华夏医学》
CAS
2012年第4期484-486,共3页
Acta Medicinae Sinica
基金
广西中医药管理局中医药科技项目(GZKZ09-52)
桂林市科技局科学技术研究与开发项目(20120105-9)
关键词
杠板归
多糖
丙酮沉淀法
正交试验
Polygonum perfoliatum
polysaccharide
acetone precipitation
orthogonal test
作者简介
黄家伟(1974-),男,广西南宁人,1998年毕业于广西中医学院,药学本科,现任南宁市青秀区中心卫生院主管中药师,从事中医药应用研究;通信作者:邹登峰,副教授,从事天然药物的开发与研究,E-mail:zdf1226@163.com。
