摘要
【目的】验证国际植物新品种保护联盟(UPOV)发布的金鱼草品种特异性、一致性和稳定性(DUS)测试指南中数量性状在我国的适应性,为建立金鱼草种质数量性状的科学评价方法、研制适应我国生态气候的金鱼草DUS测试指南奠定基础。【方法】以UPOV发布的金鱼草新品种测试指南(TG/221/1)为标准,对40份金鱼草种质开展种植试验,对植株、叶、花等部位的13个主要数量性状进行数据采集,应用SPSS软件对采集数据进行变异程度、数量性状分级和主成分分析。【结果】11个指南性状与2个非指南性状种间变异系数为16.9%~65.9%,种内变异系数为5.3%~12.2%,符合指南性状的选择标准;通过最小显著差法得到13个性状的表达状态分级,可作为金鱼草种质鉴定和DUS指南研制的参考;通过主成分分析法得到4个重要因子并新增了2个指南分组性状。【结论】通过金鱼草种质数量性状数据分析,初步确定了各性状不同分级的数值范围,验证了UPOV指南在我国的适应性并对其中花序长度的分级数量进行优化,新增主茎长度和主茎一级分枝数量作为指南分组性状候选,为我国金鱼草指南的研制提供支持。
【Objective】Applicability of the guidelines recently released by the International Union for the Protection of New Varieties of Plants(UPOV)for quantitative DUS traits determination and classification of Antirrhinum majus L.in China was examined.【Method】An experimental planting of 40 germplasms of A.majus L.was conducted following the guidelines TG/221/1 issued by the UPOV.Thirteen DUS traits of the plants,leaves,flowers,and other parts were collected to statistically analyze their variations,classes,and principal components using SPSS for the application on the plant varieties in China.【Result】The variation coefficients that met the selection criteria set by the guidelines were 16.91%-65.87%among different species and 5.29%-12.18%within a same species on 11 traits,which were specified by the guidelines,as well as two additional ones identified by this study.The expressions of these 13 traits differentiated by least significant difference could all be applied to adequately identify the germplasms and for the DUS guideline development on A.majus L in China.In addition to the 4 factors listed in the UPOV guidelines,the principal component analysis suggested 2 new criteria for the plant classification.【Conclusion】The current UPOV guidelines provide quantitative DUS traits of A.majus L.germplasms for the species classification.A close examination with a specially designed experimentation revealed additional criteria on inflorescence length,plant height,and number of primary branches on the plant for establishing guidelines applicable in China.
作者
陈宇华
陈剑锋
钟声远
钟海丰
刘中华
CHEN Yuhua;CHEN Jianfeng;ZHONG Shengyuan;ZHONG Haifeng;LIU Zhonghua(Crop Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Fuzhou Sub-center for New Plant Variety Tests,Ministry of Agriculture and Rural Affairs,Fuzhou,Fujian 350013,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2023年第8期944-952,共9页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划属公益类专项(2020R10310012)
农业农村部申请保护品种DUS测试及已知品种库维护项目(202135010400019)
福建省农业科学院青年科技创新团队项目(CXTD0078)
福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021016)。
关键词
金鱼草
DUS
数量性状
分组性状
Antirrhinum majus L.
DUS
quantitative trait
trait classification
作者简介
陈宇华(1991-),男,助理实验师,硕士,主要从事蔬菜、花卉的种质资源研究,E-mail:550189102@qq.com;通信作者:刘中华(1976-),男,副研究员,主要从事植物新品种DUS测试研究,E-mail:40464817@qq.com。
