摘要
目的建立固相萃取-超高效液相色谱(SPE-UPLC)法同时测定复方荆防颗粒中升麻素苷和5-O-甲基维斯阿米醇苷的含量。方法采用固相萃取系统对复方荆防颗粒进行处理,色谱柱为ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm),流动相为乙腈-水,梯度洗脱,流速为0.4 mL/min,检测波长254 nm,柱温为35℃。结果升麻素苷与5-O-甲基维斯阿米醇苷在5~80 mg/L范围内均有良好线性关系,线性回归方程分别为A=40 195C-248.67(r=1.0000),A=42 577C+12 782(r=1.0000);平均加样回收率为98.11%、99.28%,RSD为1.92%、1.90%(n=6)。结论 SPE-UPLC法简便准确且重现性好,可有效控制复方荆防颗粒的质量。
Objective To establish a solid phase extraction and ultra performance liquid chromatography(SPE-UPLC method for the simultaneous determination of Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside in Compound Jingfang Granules. Methods Samples were processed by the solid phase extraction system, and the separation was performed on ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm) with acetonitrile-water mobile phase for the gradient elution. The flow rate was 0.4 mL/min, the detection wavelength was 254 nm, and the column temperature was 35℃. Results The calibration curve for Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside in the range of 5-80 mg/L showed good liner. The linear regression equation for Prim-O-Glucnylcimifugin were A = 40 195 C-248.67(r =1.0000) and the linear regression equation for 4′-O-β-glucosyl-5-O-Methylvismmiside were A = 42 577 C+12 782(r =1.0000). The average recovery of Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside were 98.11% and 99.28%, and RSD were 1.92% and 1.90%(n = 6), respectively. Conclusion SPE-UPLC method is simple, accurate and reproducible and effectivly control the quality of Compound Jingfang Granules.
作者
江丽
余辉
曹月霞
龚春燕
JIANG Li;YU Hui;CAO Yuexia;GONG Chunyan(Department of Pharmacy,Institute of Dermatology,Chinese Academy of Medical Sciences and Peking Union College,Jiangsu Province,Nanjing210042,China)
出处
《中国医药导报》
CAS
2020年第1期33-37,共5页
China Medical Herald
基金
中国医学科学院医学与健康科技创新工程资助项目（2017-I2M-1-011）
关键词
复方荆防颗粒
升麻素苷
5-O-甲基维斯阿米醇苷
固相萃取-超高效液相色谱法
Compound Jingfang Granules
Prim-O-Glucnylcimifugin
4′-O-β-glucosyl-5-O-Methylvismmiside
Solid phase extraction and ultra performance liquid chromatography
作者简介
江丽(1979-),女,硕士,副主任药师,主要从事医院制剂质量标准工作;通讯作者:龚春燕(1974-),女,硕士,副主任药师,中国医学科学院北京协和医学院皮肤病医院药剂科主任,研究方向:医院药学。
